Product > Liquid Chromatography > Nano-XPLC

Nano-XPLC

Nano XPLC is an ultra-high pressure HPLC system of the nanoscale flow rate. Its ultra high pressure pump is able to reach 20,000 psi, far higher than the regular HPLC at only 5,000~7,000 psi. Users thus are free to choose longer (up to 100 cm) or nanoscale columns to conduct the complicated analysis of protein efficiently and effectively. The CVC exclusive technology of splitless nano flow rate pump allows precise, accurate, and reproducible ultra-high pressure nano-XPLC. Thanks to the patented “Closed-loop Digital Motion-Control Technology”.

Nano XPLC provides high sensitivity, stability and reproducibility. It is suitable for connection with mass spectrometer of ABI, Bruker, ThermoFisher, or Waters. It enhances the separation rate greatly to extract more components, superiorly benefiting the researches of polypeptide, protein and other bio-macromolecules. The combination of ultra-high pressure capability (20,000 psi) and nanoscale column is able to increase the precision and speed of analysis.

After three years of research, CVC develops the “Biomarkers Quantification Screening System,” an integrated system of Nano XPLC, mass spectrometer and Label-free software platform. The system enables MS to select biomarkers accurately and rapidly via the CVC ProteoMarker Q Hunter quantification and qualification of biomarker.

Instrument model Number of solvent pump Number of 10-port valve Number of 6-port valve Number of piston Number of Pressure transducer
Dual-XPLC
 4 2 6 4 4
XPLC-2D
 4 2 4 4 4
XPLC-1000
 1 0 2 1 1
XPLC-2000
 2 1 2 2 2
XPLC-3000
 3 1 4 3 3
XPLC-4000 4 2 4 4 4

Chromatographic conditions:

  • Solvent A: H2O
  • Solvent B: H2O + 1% Acetone
  • Gradient program:
    • Time:0 A :100% B :0%
    • Time:30 A :0% B :100%
    • Time:50 A :0% B :100%
    • Time:51 A :100% B :0%
  • Flow rate: 100 nL/min
  • Detector: UV@ 214 nm
  • Gradient: Regeneration Time less than 5 minites
  • Column type:15 cm X 75 µm, C18, 3 µm particles

Chromatography reproducibility of uracil, methyl benzoate, naphalene at 200 nl/min, 10 cm x 75 µm id, C18, 5 µm particles

Chromatography reproducibility of uracil, methyl benzoate, naphalene at 5 µl/min ,10 cm x 320 µm id, C18, 5 µm particles

Chromatographic conditions:

  • Solvent A: H2O + 0.1 % TFA
  • Solvent B: ACN + 0.07 % TFA
  • Gradient program:
    Time A % B %
    0 90 10
    30 40 60
    40 0 6
  • Flow rate: 200 nL/min
  • Detector: UV@ 214 nm
  • Gradient: Regeneration Time less than 15 minutes
  • 15 cm X 75 µm id C18, 5 µm particles

Chromatography of caffeine,methamphetamine,amphetamine,phenylpropanolamine and ephedrine at 10 µl/min with 15 cm X 320 µm,C18 , 3 µm particles


Comparison of chromatographic performance in terms of speed up analysis, retention time and peak area reproducibility between conventional nano-XPLC and ultra-high pressure nano-XPLC. Column was C18, 3 cm x 150 µm, 1.8 µm particles


Uracil, methyl benzoate and naphalene were analyzed at 48 µl/min, 15 cm x 320 µm, C18, 5 µm particles

5 Yeast protein were digested by trypsin and separated on 400 nl/min flow rate, 50 cm x 75 µm id, 1.8 µm particle size column and gradient on 260 min 5-23.5 % CAN, 150 min 23.5-50 % CAN and 90 min 50-80 % ACN


Mouse brain homogenates analyzed by 100 cm x 75 µm id column, 350 min 5-35 % ACN

Dual-XPLC
FeaturesFully automated dual binary gradient elution system with noLost autosampler SplitlessNearlly doubles the efficiency of LC/MS analysis Improves separation throughputSpecificationsPumpUp to 4...
XPLC-2D
Four 1/16” diameter ceramic pistons for two independent solvent composition gradient systems at a flow rate range from 10 nl/min to50 μl/min.Incorporates two patented nano-mixers with flow isolation...
XPLC-1000
one 1/16” diameter ceramic pistons for flow rate range from 10 nl/min to50 μl/min.Incorporates patented micro-flow pulse compensation technology for pulse-free solvent deliveryCan be utilized for...
XPLC-2000
Two 1/16” diameter ceramic pistons for binary solvent composition gradient at a splitless flow rate from 10 nl/min to 50 μl/min.Incorporates patented binary solvent nano-mixer with solvent cross-talk...
XPLC-3000
Three 1/16” diameter ceramic pistons for ternary solvent composition gradient at a splitless flow ,range from 10 nl/min to 50 μl/min Incorporates patented nano-mixer with flow isolation system for...
XPLC-4000
Four 1/16” diameter ceramic pistons for quaternary solvent composition gradient at a splitless flow ,range from 10 nl/min to 50 μl/min.Incorporates patented quaternary solvent nano-mixer with flow...
Description Last Updated
Dual-Core Nano-XPLC
A Novel Ultra-High-Pressure Splitless Dual Channel Nano-XPLC System for Drastic Increase in Throughtput, Resolution and Sensitivity in Drug and Complex Proteomic Sample Analysis
 2009-06-09