Features
- Fully automated dual binary gradient elution system with noLost autosampler
- Splitless
- Nearlly doubles the efficiency of LC/MS analysis Improves separation throughput
Specifications
| Pump |
Up to 4 independent solvents delivery syringe pump(s).
20,000psi control valve per pump Plus 1 to 5 time programmable Injection/switch valve(s) |
| Flow range |
10nl/min-20ul/min |
| Settable flow range |
2 nl/min-20 μl/min |
| Flow Precision |
<0.1% RSD, at 2μl/min |
| Flow Accuracy |
±1% at 500 nL/min |
| Pressure operating range |
Upto 20,000psi |
| Composition precision |
0.2% RSD, at 1000 nL/min |
| Compressibility compensation |
Automatic & Continuous with direct drive; flow rate calibration parameter; software parameter setting. |
| Recommended pH range |
1.0-12.5 |
| Gradient formation/ Profiles |
High pressure gradient with static mixer |
| Delay/Dead Volume |
<40 nl from mixer to column |
| Composition Precision |
<0.2 % RSD, at 1000 nl/min |
| Solvent Pressurizations |
10-15 psi nitrogen head pressure |
| Analog output |
0 to 1 Volt analog output for UV detector. The real time pressure monitoring and data storage. |
| Communications |
TCP/IP control. 4 additional time programmable contact closures; External Ready and Start |
| Unattended operations |
Consume only 288μl solvent per day using 75μm id nano-column(at 0.2μl/min). A 100ml solvent can last for 347days. Over-pressure auto-shut-down. Fully automated with integrated Excel AS 1000 auto-sampler and Fraction Collector/Spotter (dual functions) |
| Safety and maintenance |
Extensive diagnostics, error detection and display. Low pressure detection, over pressure detection and shutdown. Error log. |
Application
Proteomics
- Low Abundance Protein Identification & Quantification
- Complex Chemistry of Hydrophilic & Hydrophobic Samples
- High Resolution
- High-Throughput
Nano-XPLC can enhance the efficiency of quantification and qualification in proteomics
The innovative dual-core ultra-high pressure Nano XPLC greatly advances the MS application efficiency. The duration of regular nano LC analysis is about 20-60 minutes on average, the efficiency is limited when MS needs to wait till the equilibration procedure completed. Dual-core Nano XPLC allows the procedure for using dual binary gradient HPLC which can continuously pass analytes one after another to Mass Spectrometer, so MS works with one sample and when it is done, valve shifts and MS immediately works on the sample.
- Step 1
- First Binary Gradient System: analyte injected , separated, processed to MS
- Second Binary Gradient System: Preparing column and sample injection to be ready for run
- Step 2
- First Binary Gradient System: After the run ,then it proceeds to prepare column and equilibrating then being conducted valve to switch to next run
- Second Binary Gradient System: Swithes to MS and the analysis started.
- Step n
Configuration
Biomarker quantification screening system